Effects between two different point combinations of EA on traumatic spinal cord injury in neuron rehabilitation function of rats

doi:10.3877/cma.j.issn.2095-3240.2015.01.003

Abstract

Abstract:

Objective

To discuss neuron rehabilitation function and mechanism on chronic spinal cord injury (SCI) rats.

Methods

We applied modified Allen's impact method (MASCIS Impactor, precision strike)to contribute the SCI rat models. After applying EA with two different point combinations (Dazhui and Mingmen group, and Jiaji group) on chronic SCI rats, we used the BBB score to evaluate locomotion function changes of SCI rats; RT-qPCR detection and Western-blot to observe the mRNA and protein expression changes of brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3); to analyze the effects of EA intervention with different point combinations on function recovery of SCI rats in chronic phase.Statistical analysis was performed using statistical program SPSS 16.0 software . All the data are presented as mean±SD values(±s). ANOVA for comparing multiple data from different groups was used. For pairwise comparing, if homogeneity of variance was positive, LSD-t method would be choosed,while if was not, Dunnett method would be choosed. P value ＜0.05 was considered to be significant statistically.

Results

After treatments of EA with two different point combinations on SCI rats in chronic phase, BBB score at each time point showed gradually increase of locomotion function in each treatment group compared with the model group (P＜0.05), but the most significantly increase in Jiaji group(P＜0.05). For BDNF mRNA,there was significant difference among every group(F=451.8, P＜0.05),and there was increased expression of BDNF mRNA in Dazhui＆Mingmen group and Jiaji group ,compared with that in the model group(t=15.2，t=26.8, all P＜0.05)；an increased expression of BDNF mRNA was detected in Jiaji group compared with that in Dazhui＆Mingmen group(t=11.6, P＜0.05). For NT-3 mRNA,there was significant difference among every group(F=320.4, P＜0.05),and there was increased expression of NT-3 mRNA in Dazhui＆ Mingmen group and Jiaji group ,compared with that in the model group(t=14.7, t=21.2,all P＜0.05)；an increased expression of NT-3 mRNA was detected in Jiaji group compared with that in Dazhui ＆ Mingmen group(t=6.6,P＜0.05). For BDNF protein,there was significant difference among every group(F=50.3, P＜0.05),and there was increased expression of BDNF in Dazhui＆Mingmen group and Jiaji group ,compared with that in the model group(t=0.4, t=0.8, all P＜0.05)；an increased expression of BDNF was detected in Jiaji group compared with that in Dazhui ＆ Mingmen group(t=3.6, P＜0.05).For NT-3 protein,there was significant difference among every group(F=39.6, P＜0.05),and there was increased expression of NT-3 in Dazhui ＆ Mingmen group and Jiaji group ,compared with that in the model group(t=3.8, t=6.9, all P＜0.05)；an increased expression of NT-3 was detected in Jiaji group compared with that in Dazhui＆Mingmen group(t=3.1, P＜0.05).

Conclusion

EA administrations with different point combinations on SCI rats in chronic phase, greatly promoted neuronal recovery and function rehabilitation by increasing the expression of BDNF and NT-3 in local injury spinal cord,whereas, the Jiaji group had the most remarkable effect.

Key words: Spinal cord injuries, Electroacupuncture, Governor vessel, Point EX-B2(Jiaji), Brainderived neurotrophic factor, neurotrophin-3

Songhe Jiang, Haiyan Lin, Rong He, Liping Gao, Wenzhan Tu, Weijing Liao. Effects between two different point combinations of EA on traumatic spinal cord injury in neuron rehabilitation function of rats[J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2015, 04(01): 7-12.

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References 13

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组别	鼠数（只）	1d	1周	3周	7周
假手术组	8	20.00±0.00^abc	21.00±0.00^abc	21.00±0.00^abc	21.00±0.00^abc
模型组	8	0.00±0.00^de	1.00±0.00^de	5.04±0.46^de	8.04±0.46^de
大椎命门组	8	0.25±0.44^f	2.08±0.28^f	9.04±0.86^f	13.33±1.12^f
夹脊组	8	0.50±0.51	2.88±0.34	10.21±0.83	14.71±1.33
F		2.05	4.51	2.71	832.87
P		0.00	0.00	0.00	0.00

组别	鼠数（只）	1d	1周	3周	7周
假手术组	8	20.00±0.00^abc	21.00±0.00^abc	21.00±0.00^abc	21.00±0.00^abc
模型组	8	0.00±0.00^de	1.00±0.00^de	5.04±0.46^de	8.04±0.46^de
大椎命门组	8	0.25±0.44^f	2.08±0.28^f	9.04±0.86^f	13.33±1.12^f
夹脊组	8	0.50±0.51	2.88±0.34	10.21±0.83	14.71±1.33
F		2.05	4.51	2.71	832.87
P		0.00	0.00	0.00	0.00

组别	鼠数（只）	BDNF	NT-3
假手术组	8	1.26±0.37^abc	0.54±0.11^abc
模型组	8	2.94±0.57^de	1.92±0.42^de
大椎命门组	8	6.88±0.60^f	5.25±0.63^f
夹脊组	8	9.88±0.50	6.74±0.49
F		451.8	320.4
P		0.00	0.00

组别	鼠数（只）	BDNF	NT-3
假手术组	8	1.26±0.37^abc	0.54±0.11^abc
模型组	8	2.94±0.57^de	1.92±0.42^de
大椎命门组	8	6.88±0.60^f	5.25±0.63^f
夹脊组	8	9.88±0.50	6.74±0.49
F		451.8	320.4
P		0.00	0.00

组别	鼠数（只）	BDNF	NT-3
假手术组	8	0.39±0.07^abc	0.32±0.06^abc
模型组	8	0.49±0.050^de	0.42±0.06^de
大椎命门组	8	0.62±0.043^f	0.53±0.06^f
夹脊组	8	0.72±0.06	0.63±0.06
F		50.3	39.6
P		0.00	0.00

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