目的

观察针药结合对大鼠急性痛风性关节炎（AGA）模型不同时相的影响，探讨其治疗机制。

方法

取96 只Wistar 大鼠随机分为空白组、模型组、治疗组和对照组。 每组各24 只。 空白组大鼠不做任何处置，将其余三组大鼠造模成AGA 模型。模型组大鼠在造模后不做任何治疗处置，治疗组大鼠于造模后30 min 开始中药灌胃及针刺治疗，对照组大鼠于造模后30 min 开始秋水仙碱灌胃治疗。将各组大鼠分别在治疗l、3 及5 d 后采用酶联免疫吸附测定法检测关节滑膜组织中白细胞介素4（IL-4）的含量，并将其结果进行统计学分析。

结果

治疗组、对照组、模型组和空白组第1 天、第3天和第5 天细胞因子IL-4 含量组间比较，差异均有统计学意义（F=81.36、64.69、18.57，均P＜0.05）。 治疗组与对照组细胞因子IL-4 含量于治疗后第1 天和第3 天比较，差异均有统计学意义（t=4.00、5.27，均P＜0.05）；治疗组细胞因子IL-4 含量分别与模型组和空白组于治疗后第1、3 及5 天比较，差异均有统计学意义（t=3.27、4.09、5.00、12.50、9.00、2.60，均P＜0.05）。

结论

针药结合疗法对AGA 模型大鼠疗效显著。

Objective

To discuss the treatment effects of traditional Chinese medicine combined with acupuncture on different stages of acute gouty arthritis（AGA） of model rats， and its mechanism thereof.

Methods

A total of 96 Wistar rats were randomly divided into four groups: blank group， model group，treatment group and control group（n=24 for each group）. Rats were not given any treatment in blank group. The rat model of AGA was established in other three groups. Rats were given traditional Chinese medicine and acupuncture treatment 30min after modeling in treatment group. Rats were given colchicine in control group and no any treatment for rats of model group. The levels of interleukin-4（IL-4） in synovial tissue were detected by ELISA assay on 1d， 3d and 5d treatment in four groups.

Results

There were significant differences in the levels of IL-4 between four groups（F=81.36，64.69，18.57，all P＜0.05）. There was a significant difference in the level of IL-4 between treatment group and control group（t=4.00，5.27，all P＜0.05）. It was also significantly different in the level of IL-4 between model group and control group（t=3.27，4.09，5.00，12.50，9.00，2.60，all P＜0.05）.

Conclusion

There was significant therapeutic effect of Chinese herbs combined with acupuncture on AGA rats.