Effect of electroacupuncture combined with medication on expressions of TNF-α and HSP-70 in AGE rat model

doi:10.3877/cma.j.issn.2095-3240.2013.02.002

Abstract

Abstract:

Objective

To investigate the therapeutic effect of traditional Chinese medicine combined with acupuncture treatment on acute gouty arthritis（AGA）rat model during the different stages and the mechanism thereof.

Methods

Ninety-six rats were randomly divided into blank group，model group，treatment group and control group.The rat model of AGA was established in the last three groups of rats.The treatment of traditional Chinese medicine combined with acupuncture was given after 3 hours.Colchicin was given to control group.The expression levels of tumor necrosis factor-α（TNF-α）and heat shock protein（HSP）-70 in synovial tissues were detected by ELISA and immunohistochemistry methods after 1，3 and 5d treatment.Results were compared between four groups.SPSS 13.0 software was used.One-way analysis of variance was used in multiple comparison and LSD-t test was used for two-group comparison.

Results

（1）The level of TNF-α was significantly higher in model group than that in treatment group and control group（F=7.213，P﹤0.01）.There was no significant difference in the level of TNF-α between treatment group and control group（P﹥0.05）.（2）The expression level of HSP-70 was significantly higher in treatment group and control group than that of model group（F=62.378，P﹤0.01）.There was no significant difference in the level of HSP-70 between treatment group and control group（P﹥0.05）.

Conclusion

Traditional Chinese medicine combined with acupuncture treatment therapy on AGA in rat model has significant therapeutic effect in AGA rat model，and which is suitable for clinical application.

Key words: Acupuncture therapy, Drugs，Chinese herbal, Gout, Animal experimentation

Ze JIN, Jia XU, Yu-lin WANG, Jing GAO. Effect of electroacupuncture combined with medication on expressions of TNF-α and HSP-70 in AGE rat model[J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2013, 02(02): 56-59.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhzjdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-3240.2013.02.002

https://zhzjdzzz.cma-cmc.com.cn/EN/Y2013/V02/I02/56

Figures/Tables 3

References 8

［1］	陈洪岩，夏长友.简明实验动物学［M］.哈尔滨：哈尔滨出版社，2002，216.
［2］	时乐.痛风性关节炎动物模型的研究现状与展望［J］.中国实验动物学报，2006，14（1）：73-74.
［3］	田华，顾冬梅.朱良春教授治疗痛风性关节炎经验介绍［J］.新中医，2010，42（9）：132-133.
［4］	吕耀中，胡庆华.二妙丸水提取物对高尿酸血症小鼠尿酸失衡及其相关基因和蛋白水平的影响［J］.中草药，2010，41（3）：418-423.
［5］	谢建祥，黄敏员，黄国栋.白艾痛风灵免煎颗粒剂对急性痛风模型炎症因子的调节作用［J］.世界中西医结合杂志，2010，5（12）：1037-1038.
［6］	Li Z，Davis GS，Mohr C，et al.Inhibition of LPS-induced tumor necrosis factor-alpha production by colchicine and other microtubule disrupting drugs［J］.Immunobiology，1996，195（4-5）：624-639.
［7］	Villar J，Edelson JD，Post M，et al.Induetion of heat stress protein is assoeiated with decreased mortality in an animal model of aeute lung injury［J］.Am Rev Res Pir Dis，1993，147（1）：177-181.
［8］	Marber MS，Latchman DS，Walker JM，et al.Cardiae stress protein elevation 24-hours after brief isehemia or heatstress is associated with resistance to myocar-dial infaretion［J］.Cireulation，1993，88（3）：1264-1272.

组别	鼠数（只）	第1天	第3天	第5天
空白组	8	0.15±0.02	0.14±0.02	0.16±0.03
模型组	8	0.36±0.04^a	0.30±0.05^a	0.24±0.04^a
治疗组	8	0.28±0.05^abc	0.24±0.03^abc	0.20±0.04^ade
对照组	8	0.22±0.04^ab	0.21±0.03^ab	0.20±0.04^ab
F值		39.727	25.997	7.213
P值		0.000	0.000	0.001

组别	鼠数（只）	第1天	第3天	第5天
空白组	8	0.15±0.02	0.14±0.02	0.16±0.03
模型组	8	0.36±0.04^a	0.30±0.05^a	0.24±0.04^a
治疗组	8	0.28±0.05^abc	0.24±0.03^abc	0.20±0.04^ade
对照组	8	0.22±0.04^ab	0.21±0.03^ab	0.20±0.04^ab
F值		39.727	25.997	7.213
P值		0.000	0.000	0.001

组别	鼠数（只）	第1天	第3天	第5天
空白组	8	0.25±0.00	0.25±0.00	0.25±0.00
模型组	8	0.32±0.096^a	0.30±0.053^a	0.59±0.13^b
治疗组	8	0.41±0.13^bce	0.63±0.133^bde	0.91±0.13^bdf
对照组	8	0.69±0.12^bd	0.91±0.19^bd	0.91±0.13^bd
F值		30.021	80.170	62.378
P值		0.000	0.000	0.000

组别	鼠数（只）	第1天	第3天	第5天
空白组	8	0.25±0.00	0.25±0.00	0.25±0.00
模型组	8	0.32±0.096^a	0.30±0.053^a	0.59±0.13^b
治疗组	8	0.41±0.13^bce	0.63±0.133^bde	0.91±0.13^bdf
对照组	8	0.69±0.12^bd	0.91±0.19^bd	0.91±0.13^bd
F值		30.021	80.170	62.378
P值		0.000	0.000	0.000

[1]	Jiawei Zhu, Qiang Chen, Huiyang Wang, Tian'an Jiang. Development and preliminary application of abipolar radiofrequency biopsy needle in hemostasis after liver coarse needle biopsy in pigs [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(01): 69-74.
[2]	Jingjing Wang, Feng Fu, Jianwei Li, Dangli Ren, Chong Chen, Hui Liu, Hongtao Sun, Yue Tu. Effect of acupuncture on BDNF/TrkB signaling pathway after moderate traumatic brain injury [J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2023, 09(04): 199-205.
[3]	Baoqiang Dong. Clinical research progress of acupuncture and moxibustion and Tuina on sports injury [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2024, 13(03): 117-119.
[4]	Xiaohang Fu, Huiye Zhang, Ang Li, Huan Xu, Jie Li, Jianbing Yang. Theoretical analysis of " gynecological three needles" in treating postpartum pelvic organ prolapse [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2024, 13(02): 85-88.
[5]	Wenqing Wang, Xingping Li, Zhaojun Lu, Jiaqi Wang, Xuhui Wang. Progress of clinical research on the treatment of cardiac arrhythmias by acupuncture at Neiguan(PC 6) point [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2024, 13(01): 42-44.
[6]	Jinxing Cai, Guangxia Ni. Professor Ni Guangxia′s experience in treating tinnitus and deafness with "Xingnao Tonger" acupuncture method [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2024, 13(01): 39-41.
[7]	Chen Yang, Songtao Wang, Tao Guo, Jiwen Qiu. Study on the rules of acupoints selection in the treatment of vascular dementia by syndrome differentiation and acupuncture treatment [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2024, 13(01): 25-29.
[8]	Ding Luo, Xuemin Shi, Wenbin Fu. Standing on the shoulders of Giant: from " Xingnao Kaiqiao" to " Shugan Tiaoshen" —Professor Fu Wenbin′s inheritance and innovation of Academician Shi Xuemin′s academic and discipline construction experience [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2024, 13(01): 1-4.
[9]	Yuzheng Du, Lili Zhang, Yanan Zhang, Yinghui Chang. Explanation on International Standardized Manipulations of Chinese Medicine Xingnao Kaiqiao Acupuncture for Stroke [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2023, 12(04): 173-176.
[10]	Anna Jing, Bo Li, Yuzhen Wu, Ying Cui, Yuping Ma, Yuxian Li, Shiyi Jiang, Yutong Zhou. A study on the indications of Xingnao Kaiqiao acupuncture based on clinical evidence [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2023, 12(04): 167-172.
[11]	Jing Ding, Peiwen Li, Yingchun Xu. The application of Xingnao Kaiqiao acupuncture in severe neurological emergencies [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2023, 12(04): 161-164.
[12]	Lina Ning, Jie Xiong. The therapeutic evaluation research on treating dysarthria after cerebral infarction with Xingnao Kaiqiao acupuncture and tongue acupuncture therapy [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2023, 12(04): 146-150.
[13]	Zhihong Meng. Current status of research on the mechanism of Xingnao Kaiqiao acupuncture for diseases of multipel systems [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2023, 12(04): 142-145.
[14]	Yuzheng Du. Development course of Xingnao Kaiqiao acupuncture and acupuncture and moxibustion siscipline in 50 years [J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2023, 12(04): 133-137.
[15]	Meixia Du, Mengmeng Yan, Lishan Xiao, Fangfang Zhang, Caiyun Jiang, Cheng Zhao, Guodong Yao, Chunping Ning. Correlation analysis between ultrasound signs and clinical pain in patients with gout in the first metatarsophalangeal joint [J]. Chinese Journal of Diagnostics(Electronic Edition), 2024, 12(01): 31-37.

Please choose a citation manager

Content to export