目的

研究自拟方结合针刺对急性痛风性关节炎（AGA）大鼠模型不同时期的影响及其作用机制。

方法

将96只大鼠随机平均分为空白组，模型组，治疗组，对照组，后三组建立大鼠AGA模型，并在造模后3h治疗组开始针药结合治疗，对照组予以秋水仙碱口服。分别在治疗1、3和5d后分别用ELISA法及免疫组化法检测针药结合治疗对治疗组小鼠关节滑膜组织中肿瘤坏死因子α（TNF-α）含量及热休克蛋白70（Hsp-70）表达的影响，并与模型、对照和空白组对比。用SPSS 13.0软件包。采用单因素方差分析，组间比较选用LSD-t分析。

结果

（1）模型组TNF-α水平明显高于治疗组和对照组（F=7.213，P﹤0.01），治疗组和对照组TNF-α水平相当（P﹥0.05）。（2）治疗组和对照组HSP-70的表达水平明显高于模型组（F=62.378，P﹤0.01），治疗组和对照组HSP-70表达程度相当（P﹥0.05）。

结论

针药结合疗法对AGA的大鼠模型疗效显著，适合临床推广。

Objective

To investigate the therapeutic effect of traditional Chinese medicine combined with acupuncture treatment on acute gouty arthritis（AGA）rat model during the different stages and the mechanism thereof.

Methods

Ninety-six rats were randomly divided into blank group，model group，treatment group and control group.The rat model of AGA was established in the last three groups of rats.The treatment of traditional Chinese medicine combined with acupuncture was given after 3 hours.Colchicin was given to control group.The expression levels of tumor necrosis factor-α（TNF-α）and heat shock protein（HSP）-70 in synovial tissues were detected by ELISA and immunohistochemistry methods after 1，3 and 5d treatment.Results were compared between four groups.SPSS 13.0 software was used.One-way analysis of variance was used in multiple comparison and LSD-t test was used for two-group comparison.

Results

（1）The level of TNF-α was significantly higher in model group than that in treatment group and control group（F=7.213，P﹤0.01）.There was no significant difference in the level of TNF-α between treatment group and control group（P﹥0.05）.（2）The expression level of HSP-70 was significantly higher in treatment group and control group than that of model group（F=62.378，P﹤0.01）.There was no significant difference in the level of HSP-70 between treatment group and control group（P﹥0.05）.

Conclusion

Traditional Chinese medicine combined with acupuncture treatment therapy on AGA in rat model has significant therapeutic effect in AGA rat model，and which is suitable for clinical application.