目的

比较不同高脂饲料对高脂血症SD 大鼠的影响。

方法

复制高脂血症大鼠模型，根据随机数字表法分为普通饲料喂养组A 组及高脂饲料喂养组B～E 组，高脂饲料喂养组再根据配方的不同随机分为B、C、D、E 组，各组10 只，B 组使用配方Ⅰ，C 组使用配方Ⅱ，D 组使用配方Ⅲ，E 组使用配方Ⅳ，喂养3 周后经腹主动脉取血测定血清中总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）的含量。 采用SPSS 13.0 统计软件数据分析，所有数据资料符合正态分布，采用（x±s）表示，采用单因素方差分析比较A～E 组的TC、TG、HDL-C 及LDL-C 差异，采用LSD-t 检验进行组间两两比较。以P＜0.05 为差异有统计学意义。

结果

4 种高脂配方均不同程度的使SD 大鼠血清中脂代谢紊乱。（1）TC：与A 组比较，C 组、D 组及E 组均显著升高，差异具有统计学意义（P＜0.01），以C 组升高最明显；（2）TG：B 组、C 组及E 组极显著升高（P＜0.01），C 组升高最明显；（3）HDL-C：与A 组比较，B 组、C 组及D 组均极显著降低，差异具有统计学意义（P＜0.01），C 组降低最明显；（4）LDL-C：与A 组比较，B 组和C 组均极显著升高（P＜0.01），C 组与B、D、E 组组间比较显著升高，差异具有统计学意义（P＜0.01）。

结论

高脂饲料Ⅱ组（C 组）建模效果优于高脂饲料Ⅰ、Ⅲ、Ⅳ组。采用高脂饲料Ⅱ组饲喂大鼠3 周后可建立稳定的高脂血症大鼠模型。

Objective

To study the effect of different high-fat diets on hyperlipidemia in SD rat model.

Methods

The rat model of hyperlipidemia was established according？ to the method described in reference. According to random number table，SD rats were divided into ordinary diet group（A）and highfat diet group（B～E）. The high-fat diet group was further divided into B，C，D and E groups according to the different formulations（Ⅰ，Ⅱ，Ⅲand Ⅳ）of diet. The serum levels of total cholesterol（TC），triglyceride（TG），low density lipoprotein cholesterol（LDL-C）and high density lipoprotein cholesterol（HDL-C）were detected after 3 weeks. Data were analyzed with SPSS 13.0 software and expressed by（x±s）. The singlefactor analysis of variance was used to compare the results between A，B，C，D and E groups. LSD-t test was used for comparison between the two groups. P＜0.05 was considered statistically significant.

Results

The disorders in serum lipid metabolism were found in four kinds of formulations of high-fat diet groups.（1）The serum levels of TC were significantly higher in C，D and E groups compared with A group（P＜0.01）.The highest level of TC was in group C.（2）The serum levels of TG were significantly higher in B，C and E groups compared with A group（P＜0.01），and the highest level of TG was in group C.（3）The serum levels of HDL-C were significantly lower in B，C and D groups compared with A group（P＜0.01），and the lowest level of HDL-C was in group C.（4）The serum levels of LDL-C were significantly higher in B and C groups compared with A group（P＜0.01）. The serum levels of LDL-C were significantly higher in B，D and E groups compared with C group（P＜0.01）.

Conclusion The rat model was more successful in C group than that of A，B and D groups. The stable rat model of hyperlipidemia can be established after three weeks of high-fat diet in group C.