Effects of electric acupuncture acupoint recipes “lipid-lowering” on cholesterol metabolism related gene expression in hyperlipidemia rats

doi:10.3877/cma.j.issn.2095-3240.2015.04.005

Abstract

Abstract:

Objective

To observe the effect of electric acupuncture acupoint recipes“lipidlowering”on hydroxymethylglutaryl-coenzyme A (HMG-CoA), low density lipoprotein receptor (LDL-R)and cholesterol 7 alpha hydroxylase (CYP7A1) in fatty liver rat model with hyperlipidemia, and the mechanisms thereof.

Methods

Forty-four SD rats were randomly divided into two groups: control group(n=17) and model group (n=27). The rats of control group were fed standard diet and rats in model group were given high fat diet. Blood samples were collected from 7 rats of each group after 3-week treatment.Changes of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were detected and compared between two groups. The model group was randomly subdivided into control group and lipid-lowering group. Changes of TC, TG, LDL-C and HDL-C were observed after 4-week treatment in four groups. RT-PCR method was used to detect the values of HMG-CoA, LDL-R and CYP7A1 mRNA expression in liver tissue. SPSS13.0 statistical software was used for data analysis. The t-test was used in the comparison between the two groups, and single factor analysis of variance was used for data analyses of three groups. Statistically significance was considered when P<0.05.

Results

After three weeks, compared with control group, serum levels of TC, TG and LDL-C were significantly increased in model group but HDL-C were significantly decreased（t=4.09, 3.32, 5.22 and 14.57，P<0.01 or P<0.05). The model rats were hyperlipidemia status. The expression of HMG-CoA was significantly higher in model group than that of control group（P<0.01), and expression levels of LDL-R and CYP7A1 were significantly lower in model group than those of control group (t=54.52, 11.78 and 6.02,P<0.01). After 4-week acupuncture treatment, the serum levels of TC, TG and LDL-C were significantly decreased, the level of HDL-C was significantly increased, in lipid-lowering group than those of model group（t=2.67, 2.77, 2.76 and 3.74, P<0.05). The expression of HMG-CoA was significantly decreased, and expression levels of LDL-R and CYP7A1 were significantly increased in lipid-lowering group than those of model group (t=34.99, 4.18 and 12.95, P<0.01).

Conclusion

Electric acupuncture acupoint recipes“lipid-lowering” have good effects of prevention and treatment on hyperlipidemia, which are related with lowing blood lipid and reversing the expression of enzymes in liver.

Key words: Hyperlipemia, Fatty liver, Rats, Cholesterol, Metabolism

Zhongyuan Zhang, Haijun Wang, Jinling Miao, Guoxin Zhang, Laixi Ji. Effects of electric acupuncture acupoint recipes “lipid-lowering” on cholesterol metabolism related gene expression in hyperlipidemia rats[J]. Chinese Journal of Acupuncture and Moxibustion(Electronic Edition), 2015, 04(04): 172-175.

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分组	鼠数	TC	TG	HDL-C	LDL-C
正常组	8	1.43±0.25	0.40±0.15	0.93±0.14	0.63±0.14
模型组	8	1.82±0.10	0.89±0.39	0.59±0.12	1.93±0.21
t		4.09	3.32	5.22	14.57
P		0.00	0.01	0.00	0.00

分组	鼠数	TC	TG	HDL-C	LDL-C
正常组	8	1.43±0.25	0.40±0.15	0.93±0.14	0.63±0.14
模型组	8	1.82±0.10	0.89±0.39	0.59±0.12	1.93±0.21
t		4.09	3.32	5.22	14.57
P		0.00	0.01	0.00	0.00

分组	鼠数	TC	TG	HDL-C	LDL-C
正常组	10	1.58±0.18	0.35±0.11	1.00±0.13	0.50±0.09
模型组	10	1.78±0.12^a	0.57±0.21^a	0.82±0.11^a	0.79±0.11^a
降脂方组	10	1.59±0.19^b	0.37±0.09^b	0.69±0.10^b	0.57±0.15^b
F		13.60	2.72	33.49	4.30
P		0.00	0.01	0.00	0.00

分组	鼠数	TC	TG	HDL-C	LDL-C
正常组	10	1.58±0.18	0.35±0.11	1.00±0.13	0.50±0.09
模型组	10	1.78±0.12^a	0.57±0.21^a	0.82±0.11^a	0.79±0.11^a
降脂方组	10	1.59±0.19^b	0.37±0.09^b	0.69±0.10^b	0.57±0.15^b
F		13.60	2.72	33.49	4.30
P		0.00	0.01	0.00	0.00

分组	鼠数	HMG-CoA	LDL-R	CYP7A1
正常组	10	0.97±0.12	1.05±0.30	2.23±0.25
模型组	10	4.52±0.18^a	0.37±0.18^a	1.03±0.15^a
降脂方组	10	1.78±0.17^b	0.86±0.15^b	1.34±0.22^b
F		9.26	4.38	8.79
P		0.00	10.00	0.00

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